Light Sheet Fluorescence Microscope with an Optofluidic Chip

May 6th, 2021 ANDREA BASSI Politecnico di Milano

Opto-fluidic technologies integrate multiple fluidics and photonics devices in a miniaturized chip. Among many lab-on-chip applications, they can be used for optical microscopy.

The seminar will discuss the design and applications of a millimeter-scaled optofluidic device that incorporates light-sheet illumination and automatic sample delivery and scanning. This device upgrades a standard inverted microscope to a high throughput, three-dimensional, Light Sheet Fluorescence Microscope. A cylindrical lens fabricated by Femtosecond Laser Irradiation followed by Chemical Etching (FLICE) is integrated with an optical waveguide or an optical fiber on a fused silica chip. The lens is used to illuminate a single plane of the sample, at two alternating wavelengths. The samples flow in a three-dimensional fluidic network (fabricated by FLICE too) and is then imaged on a CMOS running at camera at 400kHz.

Several applications will be discussed in the seminar, together with the technological solutions for automatic sample alignment, including automatic imaging of tumor spheroids, Drosophila embryos, and high NA imaging of cancer cells.

Andrea Bassi received his Ph.D. in Physics at Politecnico di Milano in 2006. He conducted research at Beckman Laser Institute, University of California (Irvine) in 2005/2006 as Research Specialist, at Politecnico di Milano from 2009 to 2014 as Researcher, at Max Planck Institute of Molecular Cell Biology and Genetics (Dresden) in 2013/2014 as Marie Curie Fellow. Currently he is an Associate Professor at the Department of Physics of Politecnico di Milano.


All interested may join this session. Participants will be asked to register upon entry. After registering, you will receive a confirmation email containing information about joining the meeting.

L4H Seminar, May 6, 2021, 12:00. Online (Zoom)

Hosted by Dr Pablo Loza Álvarez